Appendix A Troubleshooting
Error Possible causes Troubleshooting steps
Dramatic change in PMT for one
single channel
Improper bandpass filter installed Run the ZE5-EYE process
and inspect filters. See Using
the ZE5-EYE to Confirm
Filter Choices on page 135.
Improper dichroic filter placement Run the ZE5-EYE process
and inspect filters. See Using
the ZE5-EYE to Confirm
Filter Choices on page 135.
PMT malfunctioning Contact Bio-Rad Technical
Support for help.
Mirror or filter scratched Inspect and replace if
necessary. See Working with
Optical Filter Configurations
on page 120.
Contamination of flow cell (if PMT
change is seen in FSC channel)
Follow these steps, testing
after each to determine
whether the problem has
been resolved:
1. Run the cleaning process.
See Cleaning the Sample
Line and Probe on page
324.
2. Run the Decontamination
wizard. See
Decontaminating the
System on page 325.
346 | ZE5 Cell Analyzer and Everest Software
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