Appendix A Troubleshooting
Error Possible causes Troubleshooting steps
Two or more populations are
present when expecting one
Gating inaccurate Adjust gates. See Applying
Filters (Gates) on page 236.
Target protein expressed on
multiple cells
Evaluate sample preparation
and experiment setup.
Inadequate cell preparation Ensure adequate cell
separation and preparation
because multiple cell types
or debris could be present in
a sample.
Large number of doublets in
sample
Adjust the flow rate down.
Unexpected fluorescence signal Free dye accumulating in sample
line
Run the cleaning process.
See Cleaning the Sample
Line and Probe on page 324.
356 | ZE5 Cell Analyzer and Everest Software
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