Acquisition/Event Issues
Error Possible causes Troubleshooting steps
Weak or no fluorescence Not enough antibody used during
sample preparation
Verify antibody titrations and
prepare a new sample.
Intracellular targets insufficiently
labeled
Ensure correct techniques
are used to fix cells.
Incorrect fluorophore selection Evaluate sample preparation
and experiment setup.
Poor compensation Run the compensation
process. See Adjusting
Compensation Automatically
on page 295.
Reagent old or degraded Antibody might not have been
stored in the proper
conditions (refrigerated and
kept in the dark).
Antibodies are not compatible Verify that the secondary
antibody used has been
grown against the species in
which the primary antibody
has been grown.
Lasers turned off Turn on lasers in software.
See Configuring Instrument
Settings on page 257.
Lasers misaligned Contact Bio-Rad Technical
Support.
User Guide | 357
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