100 | Chapter 3: Operation
Cassette Type Dead Volume
1 µL 1.20 mL
5 µL 4.23 mL
10 µL 7.36 mL
RAD technology single-tube cassettes have the lowest dead volume:
Type Dead Vol.
1 µL 150 µL
5 µL 530 µL
10 µL 920 µL
Use BioTek's Peri-pump Reservoir Holder on page 60 and Shorten the Dispense Cassette
Tubing on page 61 to significantly reduce the dead volume to preserve expensive reagents.
At the start of the day:
Prime the tubing to prepare for a dispense run.
1. Reload the cassette and fill the supply vessel:
l When dispensing solutions not effected by water, prime with the dispense fluid.
l When dispensing protein solutions, first prime the tubing with a buffered saline
solution to remove any traces of water in the tubing, then, prime with the dispense
fluid.
2. Press the Prime button until fluid flows into the priming trough and all visible
air bubbles have been removed.
At the end of the day:
Purge the tubing to reclaim the dispense fluid, then Prime the tubing to flush it
clean.
1. Press the Purge button until the tubing appears empty.
2. Replace the supply vessel with the appropriate rinse fluid:
l When dispensing water soluble solutions, use deionized or distilled water.
l When dispensing protein solutions, first prime the tubing with a buffered saline
solution to remove protein particles, then, prime with deionized or distilled water.
3. Press the Prime button:
MultiFlo™ FX Multi-Mode Dispenser
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