Biotek MultiFlo FX - Syringe Dispense Step

Syringe Dispense Step | 163
Syringe Dispense Step
Add a Syringe pump dispense step to the protocol:
LHC/Touch Screen
Click S-Dispense and define the parameters
Define the Dispense Step
1. Select the Syringe to dispense with:
o
A or B or Both dispensers.
o
Note: "Both" is not an option when low-density-plate-type manifolds are installed.
n Both: When both Syringe dispensers are used, they simultaneously dispense
the specified volume/well in a pattern that varies depending on the plate type.
Remember to create two prime steps, one for each dispenser, to precede the
dispense step. See Dispense Pattern with Both Syringes on page 140.
n 1536-well Flange Plates: Use "Both" Syringe dispensers when processing
1536F plates.
2. Enter the Volume to dispense per well. The valid values are 5-30,000 µL
depending on the plate type.
3. Define the Flow Rate: The rate at which the fluid is dispensed. Options range
from 1-5, 1 is the slowest, 5 is the fastest. The valid rate is volume dependent.
Lower rates are recommended for viscous fluids.
Rates are volume and plate-type dependent:
For example, rate 1 must be used when dispensing between 10-19 µL to a 96-well
plate. When dispensing 20-49 µL to a 96-well plate, you can use rates 1 or 2. And,
when dispensing 50-59 µL to a 96-well plate, you can use rates 1, 2, or 3. And so
on, as shown in these tables.
96-well plate 16-Tube 8-Tube
Volume
(µL)
Rate µL/sec/well
10-19 1 450 140
20-49 1-2 600 209
50-59 1-3 750 279
60-79 1-4 900 350
80-3000 1-5 1000 420
BioTek Instruments, Inc.