156 | Chapter 3: Operation
Cassette Type Requirement
LHC/Touch screen
Optionally, Require (a) specific cassette type to run this protocol.
l Fill the checkbox to ensure users install the correct cassette type when running this
protocol.
l Leave the checkbox empty to allow any cassette type to be used.
l For RAD technology cassettes choose both tubing and cassette type.
Note: Half-microliter (0.5 µL) dispensing requires a 1 µL cassette. The Cassette
Requirement Mode behavior is implemented whenever a 0.5 µL volume is requested.
Make sure a 1 µL cassette is installed and the Cassette Type setting matches it.
Only a 0.5 µL dispense volume can be requested, not 1.5 or 2.5 µL, for example. Perform
two dispense steps, one for the half microliter and another at a full increment to achieve
these volumes.
Pre-Dispense
When enabled, the Peri-pump primes into the priming trough immediately
before dispensing to the plate. Pre-dispense is recommended for most
applications. It normalizes the tips, to correct for evaporation, for example, to
ensure precise fluid distribution.
See Protocol Parameters Tables on page 103 for details about the remaining
parameters.
RADTip Tracking
When using the 8-to-1 bulk-dispensing cassette, you can further minimize the
chance of disturbing cells in the bottom of a well using "tip tracking." When
enabled, the chute or tip gradually rises as fluid is dispensed into the well.
Dispensing begins at the specified dispense height (Z-axis) and
moves up to a maximum height that is equal to the Plate Clearance
height.
Prerequisites:
l
Tip Tracking must be enabled in Instrument Settings
l
You must define a dispense protocol, i.e. do not use Quick
Dispense.
l The protocol's initial dispense height or Z-axis position must be
lower than the default height.
Shortcut: Make a copy of the sample protocol: P2 RAD
MultiFlo™ FX Multi-Mode Dispenser
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