142 | Chapter 3: Operation
6-well, 24-well, and so on. Manifold configurations vary
to accommodate the geography of the plate; at times
using multiple tubes in a well to accomplish the task.
Cell Wash
See how to Define a Cell Wash Protocol below.
The MultiFlo FX supports cell-based assays that require the addition and removal
of buffer solution without disrupting the cells in the wells of the microplate. Cells
are often dislodged when fluid is dispensed at too high a pressure and lost during
subsequent aspiration of the fluid from the well unless counter measures are taken.
When the Flow Rate is set to 1 or 2, the MultiFlo FX dispenses fluid to the wells
slowly enough to avoid damaging the cells.
Additional Techniques
Delay Aspiration: Also critical to cell-based assays is delaying aspiration to allow
the slower dispense process to finish before beginning fluid removal from the well.
This option, offered as part of the dispense step, is called Delay Start of Vacuum.
Adjust the Aspirate Travel Rate and Aspirate Height: when defining the aspirate
step select one of the specially designed travel rates that minimize turbulence in
the wells. Increase the aspirate height to leave more residual fluid in the wells to
protect the cell layer. Also, consider using a secondary aspiration.
Adjust the Dispense Flow Rate, Height and Position: when defining the dispense
step be sure to select one of the special Flow Rates, 1 or 2 CW. Also reposition the
dispense tubes to aim the fluid at the side of the wells to further minimize
turbulence. See Cell Wash Strategies on the facing page.
Slow down the Plate Carrier: select Instrument>General and set the Plate Carrier
Speed to Slow.
Define a Cell Wash Protocol
Adjust the volumes recommended in this procedure to meet your specific needs.
1.
Create a new protocol.
2.
Add a Prime step, especially when the lines are empty or when
changing fluids.
MultiFlo™ FX Multi-Mode Dispenser
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