Peri-pump Dispense Precision and Accuracy Tests | 223
l 8-to-1 Test: Confirms the performance of the RAD technology 8- to-1 bulk-dispensing
cassette 40 µL/well into a 24-well plate (which is 8 times the full 5 µL tubing
increments). Use the 100 µL solution, and top off the wells with an additional 1080
µL/well of deionized or distilled water to raise the fluid level for optimal reading.
Peri-pump Dispenser Test Materials
l 96-well plates: Corning
®
Costar #3590 or equivalent
l 1536-well plates: Nunc #264710, if applicable
l 24-well plates: Corning
®
Costar #3524, for RAD 8-to-1 cassette
l Precision balance with readability of 0.0001 g resolution is preferable, 0.001 g resolution
is acceptable, and capacity of 100 g minimum
l Pipettes and graduated beakers
l Microplate absorbance reader capable of dual wavelength reading at 630 and
450 (or 405) nm
l BioTek blue dye solution, PN 7773001, or equivalent to create the Peri-pump Dispense
Precision and Accuracy Test Solutions below.
n See Important Recommendations for All Liquid Tests on page 220.
Peri-pump Dispenser Precision and Accuracy Test Solutions
Unique concentrations of the test fluid are described here, each one corresponds to
a specific dispense volume. Prepare the solutions you will need to validate the
cassette types and dispense volumes used most commonly in your applications.
n The 5 µL Solution is used to make the higher volume test solutions.
1 µL Solution
Using BioTek’s 10X concentrated blue dye solution (PN 7773001), mix 5 mL of
deionized or distilled water with 8 mL of the blue dye solution.
5 µL Solution
Using BioTek’s 10X concentrated blue dye solution (PN 7773001), mix 100 mL of
deionized or distilled water with 10 mL of the blue dye solution.
10 µL Solution
Mix 25 mL of DI or dH2O water with 20 mL of the 5 µL Solution (described above).
BioTek Instruments, Inc.
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