Install RAD Technology Components | 61
Shorten the Dispense Cassette Tubing
Shortening the fluid path reduces the dead volume when dispensing, which helps
preserve expensive reagents. BioTek offers the Peri-pump Reservoir Holder on the
previous page as an accessory to the Peri-pump dispenser for this purpose. The
cassette tubing can be shortened by as much as 12 inches (30.48 cm) when using the
reservoir holder.
Recommendations for cutting the tubing:
l Prepare a clean, lint-free work area to lay out the cassette;
l Remove the Tube Organizer (not applicable to RAD single-channel cassette);
l Use a ruler as a guide to determine where to cut the tubing;
l Cut one tube at a time using a razor blade, Exacto
®
knife, or sharp scissors; make as
clean a cut as possible;
l Cut the tubing as evenly as possible, limiting the difference between the tubes to less
than 1/4" (6.35 mm) (not applicable to RAD single-channel cassette).
To may make this job easier, use the cassette’s packaging to hold the cassette parts,
keeping them out of your way.
Dead Volume Reduction: If you cut the Peri-pump tubing (and use the reservoir
holder), you can reduce the dead volume:
Standard and RAD 8-to-1 Cassettes
Cassette Std length tubing Cut tubing by 12"
1 uL 1.20 mL 0.96 mL
5 uL 4.23 mL 3.38 mL
10 uL 7.36 mL 5.89 mL
RAD Single-tube Cassettes
Cassette Std length tubing Cut tubing by 12"
1 µL 150 µL 90 µL
5 µL 530 µL 320 µL
10 µL 920 µL 555 µL
BioTek Instruments, Inc.
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