108 | Chapter 3: Operation
1536-well plate
Volume (µL) 3-3000
The 32-tube manifold flow rates do
not have minimum volumes.
The µL/sec/well for each type of
manifold, Small Bore (SB) and Large
Bore (LB), is shown:
Rate
SB LB
1
56 125
2
58 150
3
60 162
4
62 174
5
64 187
The default rate is 3.
Low-density-plate manifolds
Like the standard manifolds described above, for these plate-type-specific
manifolds the flow rate is defined as µL/sec/well and the minimum volumes are
cumulative, i.e. any rate can be used to dispense Rate 5's minimum.
Manifold
Flow/Minimum
Volume
Rate 1 Rate 2 Rate 3 Rate 4 Rate 5
6-Well Flow 560 836 1116 1400 1680
Min Vol 40 80 200 240 320
12-Well Flow 420 627 837 1050 1260
Min Vol 30 60 140 160 220
24-Well Flow 280 418 558 700 840
Min Vol 20 40 100 120 160
48-Well Flow 279 372 560 600 800
Min Vol 40 80 200 240 320
Wash Parameters Table
See Protocol Parameters Tables on page 103 for Peri-pump and Syringe
Dispenser parameters. See Plate Types Table on page 9 for default Z-axis values
or dispense heights.
Minimally, a wash step includes an aspirate step followed by a dispense step.
Select and define each option to customize the parameters for your assay.
MultiFlo™ FX Multi-Mode Dispenser
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