114 | Chapter 3: Operation
Minimum Prime Volumes for the Strip Washer
Flow rate: Wash Manifold Type
6-Well 12-Well 24-Well 48-Well 96/384-Well
1 310 315 320 360 320
2 130 180 160 360 160
3 80 180 80 360 80
4 240 180 240 360 240
5 240 240 160 360 160
6 300 240 320 360 320
7 440 300 440 420 440
8 400 420 400 420 400
9 400 480 480 600 480
10 800 780 800 630 800
11 880 870 880 900 880
Bottom Wash
Bottom washing adds an initial dispense/aspirate sequence to the protocol. Fluid
is simultaneously dispensed and aspirated to create cleaning turbulence (at the
specified height). The manifold descends to aspirate again and ends with a final
dispense to fill the wells.
Designed for strongly bound molecules in assays that require vigorous washing,
e.g. ELISA, adding a bottom wash to the protocol has shown reduced background
noise without increasing the number of wash cycles.
Adjust the Z-axis setting to position the manifold deep in the well to target the
solid phase of coated assay plates.
Bottom Wash Volume: Enter the volume of wash solution to dispense per well
during the bottom wash.
Flow Rate: Select a rate between 3-11 for best results. Cell wash options are
designed for gentle washing, while bottom wash is designed for vigorous washing.
MultiFlo™ FX Multi-Mode Dispenser
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