116 | Chapter 3: Operation
Shake/Soak Step Parameters
Select the action to perform:
l Move carrier to home position first.
Regardless of selection, the plate carrier is moved home during a stand-alone shake step
and when the total time of the shake and soak durations exceeds 1 minute. The vacuum
pump is turned off in this scenario. Moving the plate home prevents drops from
contaminating the plate.
l Shake settings:
l Duration: enter numeric values to shake the plate for up to 1 hour.
l Intensity: use the drop-down list to select a shake intensity. "Variable" cycles
through the other levels of intensity.
Choose the intensity that most closely matches your assay kit instructions.
Magnetic Bead Assays: Set the shake intensity to Slow when using a magnet
on the plate carrier.
l Soak: allows buffer/dispense fluid to remain in wells for the specified duration, i.e.
processing is delayed:
l Duration: enter values to stop processing the plate for up to 1 hour (59
minutes, 59 seconds).
n Soak is not the same as "submerge" the tips. You must define a prime step or
use the AutoPrime feature to soak the tips in the priming trough.
n Soak is equivalent to incubating the plate at room temperature or delaying the
protocol.
n Shaking and soaking the plate is also an option when defining a Wash step.
Repeat steps in a Loop
Add a Loop to the protocol to repeat one or more steps a fixed number of times.
The Loop is a step container. First, add the Loop step to the protocol and then put
the action steps between the loop start and end steps.
MultiFlo™ FX Multi-Mode Dispenser
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