Peri-pump Dispense Step | 157
8TUBE-24, and modify it for your assay. As always, experiment
with parameters to determine the optimal settings.
How to use Tip Tracking:
1. Set the Cassette Type to 5 µL: 8 tubes RAD and enable Tip Tracking.
2. Define a protocol using:
l the external Peri-pump
l a 6-, 12-, or 24-well plate
l a Z-axis height 2-4 mm above the cells/well contents
l an X-axis value that positions the tip chute as close as possible to the well
wall
n Use the Align utility to determine the X- and Z-axis.
3. Install the bulk-dispensing cassette, and the plate and you're ready to run.
Important: Remove the priming trough inserts when using the 8-to-1 RAD cassette.
The chute will collide with both the RAD priming cup and the Syringe dispenser insert if they
are left in the priming trough.
RAD Well Map
RAD technology's single-channel dispenser is ideal for partially filling a plate. It
lets you pick the individual wells to dispense to (hit-picking). Green wells are
dispensed to, white wells are skipped.
Quick Dispense Protocol Dispense Step
Press Well Map
Create/Edit the Protocol>P-Dispense, enable RAD and select
Advanced options, then Well Map
l Select a well to toggle its state: fill or skip.
l Select a column or row header to fill or skip the whole row or column.
l
In the top corner of the map, use the All options to change the fill or skip state of the
wells.
The multiple-well selection options use the state of the first well in the row, column, or
plate (A1) to determine whether to fill or skip the remaining wells.
BioTek Instruments, Inc.
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