Biotek MultiFlo FX - Fluid Delivery

280 | Chapter 6: Troubleshooting
Problem Possible Cause What To Do
optimization. page 101.
Aspirate tubes are
bent.
Gently attempt to straighten the tubes using your
fingers. Contact BioTek TAC if the tubes remain
bent.
Fluid Delivery
Problem Possible Cause What To Do
Unable to
dispense
fluid.
Inlet tube not connected. Make sure all tubing is connected properly.
Check all external tubing for kinks or clogs.
Clogged valve Run Quick Prime several times, to try to
unclog valve.
Clogged dispense tubes on
the washer manifold.
Clean the Washer Manifolds
Check-valve flow direction
is incorrect.
Make sure the flow-direction arrows on the check
valves match the actual flow direction.
Check-valves are stuck
closed.
Clean or replace the check-valves. See Clean
or Replace the Check Valves on page 204.
No wash or rinse fluid. Fill bottles with appropriate fluid. Ensure bottles
are clean and do not contain particles or organic
material.
Unable to
dispense
fluid.
System not primed. Large
air pockets in tubing.
Run W-DAY_RINSE multiple times.
Insufficient suction,
clogged tubing, or faulty
valve.
Perform Washer Maintenance; If the problem
persists, contact BioTek TAC.
Plate
overfills
(floods).
Dispense height too high.
The aspirate tubes are too
far above the wells to
prevent overflow.
Lower the dispense height (Z-axis position) in the
protocol.
Dispense flow rate too
low.
Define a higher dispense Flow Rate in the
protocol.
Aspiration tubes hit Manifold may not be properly seated or mounted.
MultiFlo™ FX Multi-Mode Dispenser