Biotek MultiFlo FX - Washer Troubleshooting; Fluid Aspiration

278 | Chapter 6: Troubleshooting
Problem What To Do
wells
Dispenser crashing
into plate
Increase the Dispense Height/Z-axis for the Syringe dispenser or
Peri-pump.
Increase the Plate Clearance Height.
Washer Troubleshooting
Review these suggestions to correct problems your washer is having:
l Fluid Aspiration below
l Fluid Delivery on page 280
l Fluid Leakage on page 282
Microplate Carrier Movement
Problem Possible Cause What To Do
Aspiration tubes
not entering
wells correctly.
Microplate not
properly seated or
strips not level.
Reseat microplate in carrier or strips in holder.
Make sure the carrier is clean.
Try a different microplate or strip holder. If the
problem is unresolved, the carrier may have to be
realigned. Contact BioTek TAC.
Aspirate tubes
position is too wide
for a movement.
Change the horizontal, X- or Y-axis aspirate
position in the protocol.
Aspirate tubes
bent.
Contact BioTek TAC.
Cell layer
disruption
Fluid sloshing in
wells disturbs cells
Slow down the plate carrier speed: set the Plate
Carrier Speed in the Instrument utilities to "Slow."
Fluid Aspiration
Problem Possible Cause What To Do
Poor or uneven
aspiration.
Insufficient or no
vacuum.
Firmly seat the waste bottle cover. Ensure tubing
is connected properly.
Check all external tubing and in-line filter for
kinks or clogs. If you are using an in-line vacuum
MultiFlo™ FX Multi-Mode Dispenser