102 | Chapter 3: Operation
immediately after each aspiration in a wash cycle and/or after the final aspiration
step.
n Secondary aspiration is also called Crosswise aspiration because it is typically
performed in a different location within the well. Reposition the tubes by
defining different X-, Y-, and/or Z-axis positions when enabling the secondary
aspiration for the most effective evacuation of the wells.
Edit the Wash step or stand-alone Aspirate step to add a secondary aspiration:
LHC Touch screen
1. Double click the step in the
protocol to open it for editing.
2. Click Advanced Options (for
Aspirate) and enable Perform
Secondary Aspirate.
3. Apply a second aspiration to the
Final Aspirate, also, for best
evacuation results.
4. Optionally, adjust the position of
the manifold as it addresses the
wells.
1. Edit the Wash step or stand-alone
Aspirate step: Protocols>Edit.
2. Select Advanced Options (for
Aspirate) and enable Perform
Secondary Aspirate.
3. Use the Align tool to define a
crosswise aspirate position.
4. Apply a second aspiration to the
Final Aspirate, also, for best
evacuation results.
For example, you may want to adjust the X- or Y- axes to get to hard-to-reach areas
of certain types of wells. Or, you may want to lower the height (reduce the Z-axis),
to better evacuate fluid.
Create or Modify a Protocol
Home> Protocols
To create or edit a protocol:
1. Select Protocols at the Home/Maintenance screen.
2. Press Create or highlight the protocol and press Edit.
3. Name:
o
New protocols: Touch the name field to open a keyboard. Enter
a unique name for the protocol.
o
Editing protocols: Press the Info button and touch the name
field to change the protocol name. This will create a new copy of
the protocol you are editing. Later you can delete the original
MultiFlo™ FX Multi-Mode Dispenser
To Next Page
Loading...
