254 | Chapter 5: Qualification
6. When the protocol is completed, carefully remove the plate. Place the plate on
the balance and record the Total Dispense Weight.
7. Read the plate in an optical reader (blank on air), using the dual-wavelength
method (630 nm - 450 nm), then print or export the results.
8. Use the Dispense Precision Test Worksheet to perform data reduction:
Tip: If you have a spreadsheet software program, enter/export all 96 values into a
spreadsheet and apply your program’s Standard Deviation function (e.g., Microsoft®
Excel’s STDEV).
a. Calculate the Standard Deviation.
b. Calculate the sum of the OD values for all wells, then divide by number of
wells, e.g. 96, to determine the Mean OD for the plate.
c. Calculate the %CV: (Standard Deviation / Mean OD) * 100.
The %CV should be <= 4.0,
except for 6-well plates the %CV should be <= 6.0.
If the %CV is greater than 3.0, one or more dispense tubes may need to be cleaned.
Remove the manifold and use the stylus to clean the dispense tube(s) giving lower-
than-average absorbance readings. When finished, re-prime the washer and retry
the test.
9. When finished, prime with deionized water to flush out the dye solution.
Evacuation Efficiency Test
n Save the plate! When this test is complete, use the plate to perform the
Shake Test for the OQ, if applicable.
n Prerequisite for plate types other than 96-Well: Create the protocols
needed to test your manifold. See Create Washer Qualification Protocols
on page 252. One time only! (Predefined protocols are provided with the
instrument qualification package (PN 1260521).
n If you tared the balance at the start of the Dispense Precision and
Accuracy Test, use the plate from that test here; skip steps 1-3.
1. Fill a supply bottle with 2 liters of deionized or distilled water. Run the
Maintenance program W-DAY_RINSE two or three times to prime the tubing
and manifold.
MultiFlo™ FX Multi-Mode Dispenser
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