Biotek MultiFlo FX - Syringe Prime Step

Syringe Prime Step | 161
Quick Prime
1. Select QUICK at the home screen.
2. Press Device to select Syringe -A or -B or -BOTH.
3. Press PRIME as many times as necessary. 5000 µL is dispensed each time.
Add a Prime step to the Protocol
See Syringe Prime Step below
Similarly, always include a Pre-dispense (or tip prime) in a Dispense/Wash
step.
Keep the tubing wet during idle time
Turn on AutoPrime for the Syringe Dispenser on page 182.
Syringe Prime Step
Select S-Prime and define the parameters to prime the Syringe pump.
Adequate priming is essential for achieving expected performance. It is a good
practice to perform this step immediately before a dispense step in the protocol.
The predefined maintenance protocols for the syringe dispensers, e.g. S-DAY_
RINSE, fully prime the system, pumping 40 mL through the tubing and pump.
The approximate dead volume for each Syringe dispenser system is 12 mL. Generally,
three times the dead volume completely primes the system. When using precious fluids,
e.g., expensive reagents, you can change the prime parameters: reduce the volume or
number of cycles specified in the predefined protocols or create your own protocols.Use
the priming trough inserts to capture expensive reagents when priming.
Define a Prime Step:
1. Select the Syringe to dispense with:
o
A or B dispenser.
1. Enter the total Volume in microliters to flush through the tubing.
2. Define the Flow Rate: The rate at which the fluid is dispensed. Options range
from 1-5, 1 is the slowest, 5 is the fastest. Lower rates are recommended for
viscous fluids.
3. Enter the Number of prime cycles.
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