Perform the Syringe Dispense Precision & Accuracy Test | 236
Syringe Dispenser Test Materials
l Microplates: 384-well plates for testing the 16-tube dispensers (which can be replaced
with 96-well plates if more applicable for your lab); 1536-well plates for testing the 32-
tube dispensers; and 96-well plates for testing the 8-tube dispensers. For the low-
density-plate-type specific manifolds, the applicable plate type is required.
l Precision balance with readability of 0.0001 g resolution is preferable, 0.001 g resolution
is acceptable, and capacity of 100 g minimum
l Pipettes and graduated beakers
l Microplate absorbance reader capable of dual wavelength reading at 630 and 405 (or
450) nm
l The test solutions: Syringe Dispenser Test Solutions below.
n See also Important Recommendations for All Liquid Tests on page
220.
Syringe Dispenser Test Solutions
20 µL Solution
o
Mix 10 mL of BioTek’s blue dye solution with 100 mL of deionized or distilled water
to create a dilution of the concentrate. Mix 160 mL of deionized or distilled water
with 20 mL of the diluted concentrate.
80 µL Solution
o
Mix 120 mL of deionized or distilled water with 40 mL of the 20 µL Solution.
1536 Solution
o
Mix 21 mL of 70% Isopropyl Alcohol with 13 mL of the 20 µL Solution and 150 mL
of DI H2O.
Perform the Syringe Dispense Precision & Accuracy Test
Prerequisites:
l Gather the required materials: Syringe Dispenser Materials above.
l Prepare the test solutions: Syringe Dispenser Test Solutions above.
l Make a copy of the applicable worksheets.
BioTek Instruments, Inc.
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