Biotek MultiFlo FX - Perform the Peri-Pump Precision and Accuracy Tests

224 | Chapter 5: Qualification
50 µL Solution
Mix 45 mL of DI or dH2O water with 5 mL of the 5 µL Solution.
100 µL Solution
Mix 40 mL of DI or dH2O water with 2 mL of the 5 µL Solution. (Use this solution
to test the RAD 8-to-1 cassette.)
1536 Solution
Mix 5 mL of 70% isopropyl alcohol with 3 mL of the 5 µL Solution and 35 mL of DI
H2O.
Perform the Peri-Pump Precision and Accuracy Tests
Prerequisite:
l Gather the required materials.
l Prepare the test solutions.
l Make a copy of the applicable worksheets. Find them on the operator's manual USB stick
in the Qualification chapter PDF.
Procedure:
1. Install the cassette to be tested.
2. Turn on the MultiFlo FX and make sure the cassette type setting is correct.
3. Turn on the balance.
4. Fill a beaker or other vessel with the test solution.
5. Define a Protocol and save it for reuse. Set the parameters based on the desired
test volume:
n A predefined protocol for the 1536 Test is shipped with the instrument and
installed on your PC when you install the LHC: QC_P-1536_DISP_TEST (P2
protocols are designed for the Secondary Peri-pump).
l
Add a dispense step to the protocol: .
l Set the Dispense Volume to match the Test:
Test Volume
1 µL 1 µL
MultiFlo™ FX Multi-Mode Dispenser