AutoPrime | 183
n Important: When using the submerge option, specify a volume that fills the
priming trough inserts with sufficient fluid to cover the tubes, e.g. 6000 µL.
And, be sure to use the priming trough inserts when the option is enabled.
Learn more about soaking the dispense tubes...
Set the Submerge Duration and fill the Submerge checkbox to enable this option.
n Remove the Peri-pump cassette or its priming trough insert when soaking the
Syringe dispenser or washer manifolds.
Turn on AutoPrime for the Washer
l Learn about AutoPrime, if you haven't already done so.
1. Select Tools>Instrument Utilities>AutoPrime in the LHC or
Instrument>AutoPrime onboard.
2. Set the AutoPrime Interval: enter the downtime interval that will trigger an
AutoPrime.
3. Fill the checkbox for the washer and press its link.
4. Set the Volume and Rate. The default values are 400 µL at rate 3. x2 - Two
dispense cycles are performed.
5. You can Submerge the manifold tubes in the fluid after the prime, if desired.
Set Duration in Hours and Minutes.
After the dispense tubes have been primed, the manifold moves down into the
priming trough that is filled with the dispensed solution. The tubes are
allowed to soak. After the specified duration, the trough is aspirated.
It takes approximately 6.5 mL of fluid to fill the priming trough insert. Be sure
to specify a volume of fluid, we recommend 3500 µL, that will cover the tubes.
n Note: the washer aspirates fluid during the prime step, but it does not fully
evacuate the priming trough insert.
Keep in mind:
l AutoPrime can be stopped when it is underway: press STOP.
l Any interaction with the instrument via the touch screen or the LHC resets
the interval clock.
l AutoPrime only runs when the Home screen is displayed.
BioTek Instruments, Inc.
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