186 | Chapter 4: Maintenance
insert.
Define the AutoPrime values to fill the priming-trough-inserts, e.g. 6000 µL, and
then submerge the tubes for several hours. When AutoPrime is enabled for both
dispense manifolds, the soak duration begins after both manifolds are primed, i.e.
both priming-trough-inserts have been filled.
More about soaking the dispense tubes...
You can soak the Syringe dispenser's two manifolds, A and B, simultaneously or
separately depending on how you define the prime routine. Two priming trough
inserts are supplied, one for each manifold. The troughs can be used to preserve
precious fluids and to soak the manifold tubes.
n Note: the washer aspirates fluid during the prime step, but it does not fully
evacuate the priming trough insert.
n Be sure to use the prime trough
inserts to submerge the tips in
fluid.
Create a Prime protocol
As an alternative to AutoPrime, BioTek recommends creating a protocol with two
prime steps to soak the Syringe dispenser's tubes:
l Do not enable the submerge feature for Syringe A. Specify a volume that fills the prime
trough insert, e.g. 6000 µL.
MultiFlo™ FX Multi-Mode Dispenser
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