Biotek MultiFlo FX - Turn on Autoprime for the Syringe Dispenser

182 | Chapter 4: Maintenance
Cassette Type Purge Volume Prime Volume
10 µL 250 500
5 µL 150 300
1 µL 50 100
Note: these volumes are per tube, except for the RADcassettes, multiply times 8
for total volume. You may want to disable AutoPrime when trying to preserve
expensive reagents.
Turn on AutoPrime for the Syringe Dispenser
LHC Touch screen
1. Select Tools>Instrument
Utilities>AutoPrime;
2. Set the AutoPrime Interval: enter the
downtime interval that will trigger an
AutoPrime.
3. Fill the Enabled checkbox for the
dispenser.
4. Set the Volume and Rate. The default
settings are 400 µL at rate 3.
5. Click Send. Wait for a confirmation
message.
1. Select Instrument
>AutoPrime;
2. Set the AutoPrime Interval:
enter the downtime interval
that will trigger an
AutoPrime (for all devices).
3. Fill the checkbox for the
dispenser and, then, press its
link.
4. Set the Volume and Rate.
The default settings are 400
µL at rate 3.
Note: The Volume dispensed is actually double the input value. The Syringe dispenser
always performs two prime cycles during AutoPrime, doubling the volume. For example,
when 400 µL is entered, the dispenser actually primes the tubing with 800 µL.
You can Submerge the dispense tubes in the fluid after the prime, if desired. This
requires filling the priming trough inserts, one for each syringe. Each reservoir
holds approximately 6.5 mL.
After the dispense tubes have been primed, the manifold moves down into the
priming trough inserts. When both Syringes are enabled, the soak duration
begins after both dispensers are primed.
MultiFlo™ FX Multi-Mode Dispenser