Washer Qualification Liquid Tests | 251
OD of all wells on the plate. It is assumed that 1 mg = 1 µL of fluid for this
calculation.
Washer Qualification Test Materials
l One new microplate per test to be performed:
Microplate Type
Flat-bottom 96-well plates, Corning® Costar #3590 or equivalent
Flat-bottom 384-well plates, Corning Costar #3574 or equivalent
Flat-bottom 48-well plates, Corning Costar #3548 or equivalent
Flat-bottom 24-well plates, Corning Costar #3524 or equivalent
Flat-bottom 12-well plates, Corning Costar #3513 or equivalent
Flat-bottom 6-well plates, Corning Costar #3516 or equivalent
l Precision balance with minimum capacity of 100 g and readability of 0.001 g
resolution
l Pipettes and graduated beakers
l Microplate absorbance reader capable of dual wavelength reading at 630/450
nm
l Liquid Test Worksheets at the end of this chapter for recordingdata and
results
l Deionized or distilled water
l Test solutions described below
n These volumes are sufficient for performing the dispense tests and standard
and diagnostic Evacuation Efficiency tests with enough fluid remaining to re-
run a test, if necessary.
Test Solutions
Solution #1: Buffer Solution
Pipette 1 mL Tween 20 into 1 liter (1000
mL) of deionized or distilled water and
mix well.
or Pipette 10 mL of BioTek Wetting Agent* into
1 liter of deionized or distilled water and
mix well.
BioTek Instruments, Inc.
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