178 | Chapter 4: Maintenance
Daily Maintenance
Daily maintenance involves flushing the washer and dispensers with an
appropriate reagent or deionized water throughout the day. Routine rinsing helps
to prevent the aspirate and dispense tubes from clogging between runs. Flushing
the devices with deionized water is recommended at the end of the day for most
applications.
The recommended rinsing frequency depends on the solutions currently in use:
l When a solution containing surfactant is used throughout the day, perform
the rinsing procedure when the device is idle for more than 3 hours.
l When the solution does not contain surfactant, consider rinsing at least once
an hour.
n The type of hardware also affects rinsing frequency. The 32-tube dispense
manifolds require more diligence to keep them clog-free.
Run these protocols and enable AutoPrime to satisfy the daily maintenance
requirements:
l W-DAY_RINSE
l S-DAY_RINSE_A&B
l P-#UL_CASS_RINSE (# represents the cassette type)(P2 protocols are for the
secondary Peri-pump)
Make sure the supply bottles contain sufficient rinse solution and that the waste
bottles are empty before running the protocols.
Also see the additional maintenance procedures required when dispensing protein
solutions: Removing Protein Residuals and Fungi Growth on page 187.
AutoPrime
AutoPrime automatically conditions the dispense tubes, priming them with the
specified volume, after a user-specified amount of idle time. See AutoPrime on the
facing page.
l Press the STOP button to interrupt the AutoPrime routine when it is
underway.
l Any interaction with the instrument via the touch screen or the LHC resets
the interval clock.
MultiFlo™ FX Multi-Mode Dispenser