Soak the manifold tubes in cleaning fluid | 185
Soak the manifold tubes in cleaning fluid
Keeping the dispense and aspirate tubes in a wetted condition is required to keep
them clog free. Soak the tubing in deionized or distilled water or a cleaning fluid
whenever the device is not being used to ensure trouble-free performance.
Prime Step
1. Fill a supply bottle with a cleaning agent.
2. Click W-Prime or S-Prime and define parameters to fully prime the tubing, for
example:
Washer Syringe Dispenser
Volume = 5000 µL Volume = 8000 µL
Flow Rate = 5 Flow Rate = 5
Submerge tips in fluid after prime: enable option and set Duration, up to 24
hours.
n For the manifolds: Use the priming trough inserts to submerge the tips.
n Note: the aspirate manifold does not completely evacuate the priming trough
insert.
n Overnight Maintenance: You can turn off the instrument after the tips are
submerged to leave them soaking in the priming trough for an extended
duration.
3. Click Run. Save the program with a memorable name for future use.
AutoPrime
Enable AutoPrime to ensure the tubing is soaked during downtime intervals.
It is especially important when using the 32-tube SB dispense manifolds to keep
the tubes wet to prevent clogs. One option is to modify the AutoPrime parameters
used for idle periods during a regular work day to soak the tubing for longer
periods overnight and on the weekends.
n Be sure to use the priming trough inserts when the submerge feature is
enabled. And, remove either the Peri-pump cassette or its priming trough
BioTek Instruments, Inc.
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