235 | Chapter 5: Qualification
Syringe Dispenser Liquid Tests
Dispense Precision and Accuracy Specifications
n Important: For IQ/OQ/PQ testing purposes 1.0% tolerance has been added
to some of the published specifications for Precision %CV to accommodate
variations in test solutions, off-peak wavelengths, reader errors, and pipette
errors. % Accuracy Error is calculated for the dispense volume specified in the
respective test procedure in adherence to the published specifications.
Test #
Plate Type-Manifold Precision Accuracy
2 384-well plate < 3% CV @ 80 µL/well < 1.25% @ 80 µL/well
1 16-tube manifold < 6% CV @ 20 µL/well < 5% @ 20 µL/well
2 96-well plate < 3% CV @ 160 µL/well < 1.25% @ 160 µL/well
1 8- & 16-tube manifold < 6% CV @ 40 µL/well < 5% @ 40 µL/well
3 1536-well plate < 12% CV @ 6 µL/ well < 5% @ 6 µL/well
32-tube manifold
Low-Density-Plate-Specific Manifolds
Dispense Precision
Refer to the values below for dispense precision when measured in full plates with the
specified volume of deionized water with 0.1% Tween 20 and FD&C#1 blue dye solution
and read at 630 nm/405 nm.
Plate Type Performance Volume (µL/well)
6-Well ≤6.0% CV 5560
12-Well ≤4.0% CV 2240
24-Well ≤4.0% CV 1120
48-Well ≤4.0% CV 560
Dispense Accuracy is measured gravimetrically when dispensing any volume of room-
temperature deionized water using Flow Rate 2. For all plate-specific manifolds, dispense
accuracy shall be 2 µL or 1%, whichever is greater.
MultiFlo™ FX Multi-Mode Dispenser
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