Biotek MultiFlo FX - Syringe Movement; Peri-Pump Troubleshooting

Troubleshooting | 277
Syringe Movement
Problem Possible Cause What To Do
Syringe
position
error.
Syringe movement is blocked. Ensure the 26-pin high-density cable shipped
with the Syringe is connected to the MultiFlo
FX’s rear panel.
Contact BioTek TAC.
Syringe piston not seated all the
way to the bottom before
fastening set screw.
Reinstall the syringe piston and syringe
head.
Peri-pump Troubleshooting
Problem What To Do
Fluid stream
missing wells
Check Tip Holder, make sure it is properly seated in the dispense
arm.
Select the correct Plate Type.
Fluid splashing out
of the wells
Select the correct Plate Type.
Reduce the Flow Rate.
Lower the Dispense Height.
Uneven dispensing Make sure all cassette components are properly seated in their
respective positions.
Tips are clogged. (See Unclog the Dispense Tips on page 194.)
Recalibrate the Peri-pump Dispense Cassette on page 211.
Replace Peri-pump Dispense Cassette Tubing on page 210.
Dispenser skipping
columns
Check/define the plate map, i.e. See also Advanced Dispense
Options for Peri-pump.
Tips clogging Filter the dispense fluid to 50 microns before dispensing.
Replace the tubing.
Viscous fluids
sticking to tips
Vary the Flow Rate: experiment with different flow rates to
determine which setting best forces fluid to break from the tip.
Cannot
communicate with
computer
Check the cabling.
Select the correct COM Port.
Turn on dispenser; display Main Menu.
Foaming in the
Reduce the dispense step’s Flow Rate.
BioTek Instruments, Inc.