Decontamination | 207
Lab coat
Biohazard trash bags
Clean cotton cloths
Step-by-Step Decontamination Instructions:
l Decontaminate Exterior Surfaces below
l Decontaminate Tubing and Manifold on the next page
l Alternate Decontamination Procedure for Tubing and Manifold on page 1
Decontaminate Exterior Surfaces
Caution!Be sure to check the percentage NaClO of the bleach you are using; this
information is printed on the side of the bottle. Commercial bleach is typically 10%
NaClO; in this case, prepare a 1:20 dilution. Household bleach is typically 5% NaClO; in
this case, prepare a 1:10 dilution.
The bleach solution is caustic; wear gloves and eye protection when handling.
1. Turn off the instrument and disconnect the power cord. Empty the waste bottle.
2. Unload the Peri-pump cassette and the prime trough inserts, and remove the
Syringe dispenser manifold and tubing, if applicable.
3. Autoclave the cassette and other autoclavable components.
4. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach).
As an alternative, 70% isopropyl alcohol (or 70% ethanol) may be used if the
effects of bleach are a concern.
n Isopropyl alcohol is not recommended for removing proteins (such
as bovine serum albumin).
5. Moisten a cloth with the bleach solution or alcohol. Do not soak the cloth.
l Wipe the touch screen (do not soak). Wipe again with a clean cloth moistened with
deionized or distilled water. Dry immediately with a clean, dry cloth.
l Wipe the plate carrier, top surface of the instrument’s base, supply bottles and
tubing, and all exposed surfaces of the instrument.
6. Wait 20 minutes. Moisten a cloth with DI or distilled water.
BioTek Instruments, Inc.
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