252 | Chapter 5: Qualification
* BioTek Solution #1 100X Concentrate Wetting Agent 125 mL (PN 7773002)
contains 10% Tween 20 in deionized water and 0.01% Sodium Azide as a
preservative.
SOLUTION #2: Residual Test Solution
Mix 100 mL of Solution #1 with 0.050
grams of FD&C #1 blue dye.
or Mix 90 mL of Solution #1 with 10 mL
of BioTek Blue Test Dye*.
* BioTek Solution #2 10X Concentrate Blue Test Dye 125 mL (PN 7773001)
contains 5 grams per liter FD&C Blue #1, 0.1% Tween 20 in deionized water and
0.01% Sodium Azide as a preservative.
SOLUTION #3: Dispense Precision and Accuracy Solution
Mix 1180 mL of deionized or distilled water with 20 mL of Solution #2.
Create Washer Qualification Protocols
1. Select Maintenance at the Home screen, and then Define.
2. Press Create.
3. Press the Name field and assign a unique name, e.g. 6-Well
Dispense Test.
4. Plate Type: select the appropriate plate type for the test and
press OK.
5. Add steps with the recommended parameters (shown below):
l Add a W-Dispense step for the Dispense Precision and
Accuracy test and W-Aspirate step for the Evacuation
Efficiency test.
l Press Advanced Options to define the Z-axis, Pre-Dispense,
and other parameters.
6. Press Save to save the protocol for future use.
Dispense Precision and Accuracy Protocols
Parameters 6-Well 12-Well 24-Well 48-Well 384-well
Volume (µL/well) 5560 2240 1120 560 80
MultiFlo™ FX Multi-Mode Dispenser
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