Washer Qualification Liquid Tests | 253
Parameters 6-Well 12-Well 24-Well 48-Well 384-well
Flow Rate 5 5 5 5 5
Z-axis/Dispense
Height
465 460 452 460 333
Pre-dispense
For all manifold types enable and set its Volume to 300 µL and
Number of cycles to 2.
Retain default values for all other parameters.
Evacuation Efficiency Protocols
Parameters 6-Well 12-Well 24-Well 48-Well
Travel Rate 0 1 1 1
Z-axis 45 51 51 42
X-axis 350 222 150 96
Retain default values for all other parameters.
Dispense Precision and Accuracy Test
n Find a list of the supplies you will need at Materials on page 251.
n Save the plate! When this test is complete, use the filled plate to perform the
Evacuation Efficiency Test.
n Prerequisite for plate types other than 96-Well: Create the protocols
needed to test your manifold. See Create Washer Qualification Protocols
on the previous page. One time only! (Predefined protocols are provided
with the instrument qualification package (PN 1260521).
1. Fill a supply bottle with 2 liters of deionized or distilled water and run the W-
DAY_RINSE protocol once or twice to flush the fluid lines and manifold.
2. Fill a supply bottle with 1200 mL of Solution #3.
3. Run W-DAY_RINSE again to prime the washer with the solution.
4. Place a new microplate on the balance and zero the balance.
5. Place the plate on the carrier and run the QC_96_DISP_TEST or the protocol
you created for your manifold. The protocol dispenses solution to each well of
the plate. It does not evacuate the solution.
BioTek Instruments, Inc.
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