Biotek MultiFlo FX - Pre-Dispense

Wash Parameters Table | 113
Rate mm/sec
7 CW 30.0
CW rates are specially designed travel rates that minimize turbulence in the wells
for Cell Wash protocols. 6CW is recommended for cell-based and magnetic-bead
assays. Alternatively, use 7CW if loosely adherent cells are damaged by rate 6CW,
to further reduce turbulence in the wells. Expect a larger residual volume in the
wells when using rate 7CW. Exception: choose rate 0CW when using 6-, 12-, and
24-well plates.
Standard rates (1-5) show two speeds as they approach the well because they slow
down to 1 or 2 mm/sec before reaching the aspirate height to provide more time to
aspirate the fluid, improving evacuation. Conversely, the CW rates do not change
speeds, they move into and out of the well as quickly as possible to limit
turbulence.
Pre-Dispense
Wash and Dispense Step option
Also called Tip Priming.
About the Pre-Dispense Options
Enable the Pre-dispense options to condition or normalize the dispense tubes
before dispensing fluid. This is recommended for precise dispensing to mitigate
evaporation or any potential fluid imbalance at the ends of the tubes.
The pre-dispense options are not intended to fully prime the tubing, for example,
when changing fluids. Be sure to fully Prime the device to remove all air from the
tubing before commencing a dispense step. Use the Pre-dispense feature to quickly
prime the tubes or tips between runs.
When a Pre-dispense is enabled, the MultiFlo FX moves the plate to the home
position to allow the manifold to dispense into the priming trough.
How to condition the tips before dispensing
l In a stand-alone Dispense step: select the Advanced Options link and fill the Pre-
dispense checkbox;
l In a Wash step, select the Show Wash Options link and fill the Pre-dispense checkbox.
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