237 | Chapter 5: Qualification
Test Protocols
Two predefined QC protocols are provided for qualifying the 32-tube dispensers.
Skip the protocol development steps in the procedure when testing these
dispensers:
Onboard Name Description
SA-1536_DISP_TEST Dispense precision test protocol for 32-tube Syringe A
manifold.
SB-1536_DISP_TEST Dispense precision test protocol for 32-tube Syringe B
manifold.
These predefined protocols dispense 6µL/well into 512 wells of a 1536-well
plate (columns 2, 4, 19-30, 45, 47). For speed, efficiency and to reduce the
amount of alcohol needed, the test is designed for visual inspection of the two
columns at each end of the plate, while the block of columns at the center of the
plate is used for evaluating dispense precision.
Procedure:
Standard Syringe Manifolds: Perform two tests for each syringe using the
applicable plate type and dye solutions. Plate-Type-Specific Manifolds: Perform
one test for each syringe using the applicable plate type and the 80 µL solution.
Standard Syringe Manifolds Low-Density-Plate-Type-Specific
Manifolds
384-well for the 16-tube manifolds (unless
only 96-well plates are used in your lab),
1536-well for the 32-tube manifolds, and 96-
well for the dual 8-tube manifold.
6-, 12-, 24-, or 48-well plates supported by
the manifold.
1. Prepare the syringe dispenser to be tested:
Standard Syringe Manifolds Plate-Type-Specific Manifolds
l Test 1: Use the 20 µL solution
l Test 2: Use the 80 µL solution
l Test 3: Use the 1536 solution
l Plate-Type Test: Use the 80 µL solution
2. Prime the Syringe using your preferred method: run S-DAY_RINSE_A (or B) or
use the Quick Dispense menu to remove any air bubbles from the system.
3. Create and save protocols for the tests, two for each Syringe, A and B, as
follows, except only one test per manifold is required for plate-type-specific
MultiFlo™ FX Multi-Mode Dispenser
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