Perform the Syringe Dispense Precision & Accuracy Test | 238
manifolds:
Skip these protocol development steps for the 32-tube dispensers. And,
because you will save the protocols, you only need to create them one
time for the other manifolds.
1. Define a Dispense step for each test for each manifold, A and B:
Standard Syringe Manifolds
Manifold
Type
Plate
Type
Volume
(µL/wel
l)
Flow
rate
Pre-dispense (2 cyc)
Test 1: 16-Tube 384 20 2 20
8-Tube 96 40 2 40
Test 2: 16-Tube 384 80 2 80
8-Tube 96 160 2 160
Plate-Type Specific Manifolds
Parameters 6-Well 12-Well 24-Well 48-Well
Volume (µL/well) 5560 2240 1120 560
Flow Rate 5 5 5 5
Z-axis/Dispense Height 465 460 452 460
Pre-dispense (2 cycles) 320 220 160 320
2. Save the protocol.
4. Place a clean, empty microplate on the balance and tare the balance.
5. Place the microplate on the carrier and run the protocol (created in step 3 or
predefined).
6. Place the plate on the balance and record the Total Dispense Weight. This value
will be used to calculate the % Accuracy Error.
7. For Test 1 Only: Use a calibrated hand pipette or the Peri-pump to dispense
deionized water on top of the dye solution in the wells.
l 384-well: Pipette 60 µL/well (resulting in 80 µL/well)
l 96-well: Pipette 120 µL/well (resulting in 160 µL/well)
BioTek Instruments, Inc.
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