239 | Chapter 5: Qualification
8. Shake the plate using the MultiFlo FX, an orbital shaker or in a microplate
reader for 15 seconds, or lightly tap the side of the plate with your finger to
agitate the contents of the wells.
9. Read the plate in an absorbance reader using the dual-wavelength method, to
reduce the influence of scratches and foreign particles that could be in the well.
See the recommended wavelengths. Print or export the results.
10. Calculate and report the Mean absorbance, Standard Deviation, and the %CV for
the wells under test. %CV = (Standard Deviation ÷ Mean) * 100.
11.
The % Accuracy Error calculation is:(Actual Weight - Expected Weight) ÷
Expected Weight x 100
Subtract the expected dispense weight (see below) from the Actual (Total)
Dispense Weight (from step 5), and divide the result by the expected weight.
Multiply the result by 100.
The Expected Dispense Weight is the volume dispensed per well in mL
multiplied by the number of wells dispensed. For example, if 40 μL is
dispensed to 96 wells, the expected weight is 0.040 x 96 = 3.84 grams. We
have calculated some expected dispense weights for you:
Test # of wells
Volume
μL/well
Expected Weight
Test 1: 384 wells 20 7.68 grams
96 wells 40 3.84 grams
Test 2: 384 wells 80 30.72 grams
96 wells 160 15.36 grams
Test 3: 512 wells (of a 1536-well plate) 6 3.012 grams
Plate-Type Specific Tests
6 Well 5560 33.36 grams
12 Well 2240 26.88 grams
24 Well 1120 26.88 grams
48 Well 560 26.88 grams
MultiFlo™ FX Multi-Mode Dispenser
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