Washer Qualification Liquid Tests | 255
2. Place a new 96-well microplate on the balance and zero the balance.
3. Pipette or dispense specified volume of Solution #1 into each well of the
microplate:
Plate
Type
Volume
(µL/well)
96 Well 300
6 Well 5560
12 Well 2240
24 Well 1120
48 Well 560
4. Place the plate on the carrier and run the W-96_EVAC_TEST protocol or the
protocol you created for your manifold.This protocol evacuates the wells,
leaving a small amount of residual fluid.
5. When the program is completed, remove the plate and weigh it immediately
because evaporation will affect the results.This is the Total Residual Weight in
grams.
6. Visually inspect the plate and note if any wells appear to have considerably
more liquid than others.
7. Use the Evacuation Efficiency Test Worksheet to perform data reduction:
o
Divide the Total Residual Weight by number of wells to find the Mean
Residual Weight.
o
The Mean Residual Weight should be <=.
Plate Type
Expected Mean
Residual Weight
96 Well 0.002 g
6 Well 0.6 g
12 Well 0.15 g
24 Well 0.05 g
48 Well 0.025 g
If the Mean Residual Weight is greater than expected, or if one or more wells
appear to have much more liquid than the others, the washer failed the test.
BioTek Instruments, Inc.
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