Protocol Parameters Tables | 107
Step Option Description/Values range
Default
values
vertical axis) can be adjusted to improve
performance. Default values are plate type
dependent, and position the manifold at least 1 mm
above the plate.
Pre-
dispense:
When enabled, dispenses into the priming trough
immediately before filling the plate. Pre-dispense is
recommended for most applications. It normalizes the
tips to ensure precise fluid distribution. Set volume
and number of pre-dispenses.
10 µL
2 cycles
Syringe Dispenser Flow Rates:
Rates are volume and plate-type dependent:
For example, rate 1 must be used when dispensing between 10-19 µL to a 96-well
plate. When dispensing 20-49 µL to a 96-well plate, you can use rates 1 or 2. And,
when dispensing 50-59 µL to a 96-well plate, you can use rates 1, 2, or 3. And so
on, as shown in these tables.
96-well plate 16-Tube 8-Tube
Volume
(µL)
Rate µL/sec/well
10-19 1 450 140
20-49 1-2 600 209
50-59 1-3 750 279
60-79 1-4 900 350
80-3000 1-5 1000 420
384-well plate
Volume
(µL)
Rate µL/sec/well
5 -9 1 225
10-24 1-2 300
25-29 1-3 375
30-39 1-4 450
40-1500 1-5 500
n Note: For 16-channel syringes the µL/sec/well rate accounts for 2 tubes/well
when addressing 96-well plates and one tube/well for 384-well plates.
BioTek Instruments, Inc.
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