106 | Chapter 3: Operation
Syringe Dispenser Parameters
Step Option Description/Values range
Default
values
Prime
Flow rate: 1-5 (See dispense step description below) 5
Volume: 80-8000 µL. To remove air from the tubing. 5000
Syringe: A or B A
Cycles: Number of prime cycles to perform 2
Pump
delay:
0-5000 msec. 0
When dispensing highly viscous fluids, the tubing's
check valves perform more slowly. Delaying the
syringe pump sufficiently to allow the specified
amount of fluid to pass through the check valves
before being pumped into the syringe has been shown
to improve dispense accuracy.
Begin by setting the delay to 500 msec. Experiment
with different settings to determine the optimal value
for your fluid.
Submerge
tips:
To soak dispense tubes in the priming fluid for a
specified duration for cleaning or maintenance
purposes. If yes, set duration, up to 24 hours, in
minutes. See Syringe Prime Step on page 161 to
learn more.
0
Dispense
Flow rate: Rates 1-5 are dependent on the volume and plate
type, except for 1536-well plates. See below.
2
Dispense
Volume:
5-30,000 µL depending on the plate type. 10
Syringe: A or B or Both A
Columns: Select the plate type, and optionally, limit the columns
dispensed to.
96
Pump
delay:
(Same as Prime step description above.)
Position: X- and Y- horizontal axes, and dispense height (Z- or
MultiFlo™ FX Multi-Mode Dispenser
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