Protocol Parameters Tables | 105
Step
Option Description/Values range
Default
values
d?:
Plate
type:
Select the plate type, and optionally, limit the
columns dispensed to. For high density plates,
skipping certain "Rows" sections is supported, see 8-
Channel Dispenser Dispense Pattern on page 139.
96
Position: X- and Y- horizontal axes, Z- height (vertical) axis
can be adjusted to improve performance. X-and Y-
axes default to 0 steps for all plate types.
Z = plate
type
dependen
t
Pre-
dispense:
Also called "tip priming," pre-dispense normalizes the
tips to ensure precise fluid distribution. Pre-dispense
is recommended for most applications. Set volume
and number of pre-dispenses, except, when
dispensing 0.5 µL, the volume is preset: 0.5 µL for 4
cycles by default.
10 µL
2 cycles
Purge To preserve fluid in the tubing by pumping it back into
the supply vessel, i.e. reverses the flow direction.
Volume: 1-3000 µL 300
Duration: 1-300 seconds 3
Note: *Half-microliter (0.5 µL) dispensing requires a 1 µL cassette. The Cassette
Requirement Mode behavior is implemented whenever a 0.5 µL volume is requested.
Make sure a 1 µL cassette is installed and the Cassette Type setting matches it.
Only a 0.5 µL dispense volume can be requested, not 1.5 or 2.5 µL, for example. Perform
two dispense steps, one for the half microliter and another at a full increment to achieve
these volumes.
Important: Remember that the Peri-pump, the Syringe dispenser and Washer manifolds
travel on the same dispense arm. Keep this in mind when adjusting the Z-axis or
Dispense Height of any dispenser. A too low setting can cause a dispenser to crash into
the plate.
Uninstall the dispense cassette, if it is not required by a protocol, to prevent collisions
when trying to position the Syringe or Wash dispenser as close as possible to the wells.
And/or, consider increasing the Plate Clearance Height setting to increase the travel
height.
BioTek Instruments, Inc.
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