104 | Chapter 3: Operation
Step
Option Description/Values range
Default
values
Prime To remove air from the tubing.
Volume: 1-3000 µL 300
Duration: 1-300 seconds 3
Dispense
Volume The per-well volume to dispense: 10
Up to
30,000 µL
per well is
supported.
1 µL cassette = 1 - 50 µL and 0.5 µL (HLF-D) for some
models*
5 µL cassette = 5 - 2500 µL
10 µL cassette = 10 - 3000 µL
Flow rate: The rate, μL/second/tube, that fluid is dispensed for
each cassette type:
Cassette Type 1 μL 5 μL 10 μL
(μL/sec/tube)
Low 50 120 140
Medium 60 140 160
High 64 160 180
RAD technology: Single-channel cassette flow rates
match the standard cassettes and the 8-to-1 cassette
matches the 10 µL flow rates.
When dispensing 0.5 µL with the 1 µL cassette the
average flow rate values are:
1 μL Cassette 0.5 μL Dispense
(μL/sec/tube)
Low 52
Medium 54
High 56
High
Cassette
type
require
To require a specific cassette type for this protocol. If
yes, select the type, if no, select ANY. See Peri-
pump Settings to learn more.
ANY
MultiFlo™ FX Multi-Mode Dispenser
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