Biotek MultiFlo FX - Page 244

222 | Chapter 5: Qualification
Tests – Solutions
Cassette Types
1 µL 1 µL 1536 5 µL 10 µL
1 µL √ √
5 µL √
10 µL √ √
50 µL √
100 µL √
1536 √
8-to-1 √
l 1 µL Test:Confirms the performance of the 1 µL cassettes when dispensing a single
aliquot (1/4 turn of pump) into each well of the plate. It dispenses 1 µL into each well
using the 1 µL Solution, and requires an additional 150 µL of deionized or distilled
water to raise the fluid level for optimal reading.
n A single aliquot for a cassette type is the smallest volume unit recommended
for it. 1 µL for the 1 µL cassette, 5 µL for the 5 µL cassette, and 10 µL for the
10 µL cassette (except that later model instruments can dispense 0.5 µL/well
using a 1 µL cassette).
l 5 µL Test: Confirms the performance of the 5 µL cassettes when dispensing a single
aliquot (1/4 turn of pump) into each well of the plate. It dispenses 5 µL into each well
using the 5 µL Solution, and requires an additional 150 µL of deionized or distilled
water to raise the fluid level for optimal reading.
l 10 µL Test: Confirms the performance of the 1 µL cassettes when dispensing 10 aliquots
(2 1/2 turns of pump) and the 10 µL cassettes when dispensing a single aliquot (1/4 turn
of the pump) into each well of the plate. It dispenses 10 µL into each well using the 10
µL Solution, and requires an additional 100 µL of deionized or distilled water to raise
the fluid level for optimal reading.
l 50 µL Test: Confirms the performance of the 5 µL cassettes when dispensing 10 aliquots
(2 1/2 turns of pump) into each well of the plate. It dispenses 50 µL into each well using
the 50 µL solution, and requires an additional 100 µL of deionized or distilled water to
raise the fluid level for optimal reading.
l 100 µL Test: Confirms the performance of the 10 µL cassettes when dispensing 10
aliquots (2 1/2 turns of pump) into each well of the plate. It dispenses 100 µL into each
well using the solution called 100 µL solution, and requires an additional 50 µL of
deionized or distilled water to raise the fluid level for optimal reading.
l 1536 Test: Confirms the alignment of the tips; that the cassette is firing straight into
the wells. Dispenses 6 µL into columns 2, 4, 19-30, 45, 47 of a 1536-well plate using the
"1536 solution." Also requires performing the 1 µL Test described above.
MultiFlo™ FX Multi-Mode Dispenser