Biotek MultiFlo FX - Page 186

164 | Chapter 3: Operation
384-well plate
Volume
(µL)
Rate µL/sec/well
5 -9 1 225
10-24 1-2 300
25-29 1-3 375
30-39 1-4 450
40-1500 1-5 500
n Note: For 16-channel syringes the µL/sec/well rate accounts for 2 tubes/well
when addressing 96-well plates and one tube/well for 384-well plates.
1536-well plate
Volume (µL) 3-3000
The 32-tube manifold flow rates do
not have minimum volumes.
The µL/sec/well for each type of
manifold, Small Bore (SB) and Large
Bore (LB), is shown:
Rate
SB LB
1
56 125
2
58 150
3
60 162
4
62 174
5
64 187
The default rate is 3.
Low-density-plate manifolds
Like the standard manifolds described above, for these plate-type-specific
manifolds the flow rate is defined as µL/sec/well and the minimum volumes are
cumulative, i.e. any rate can be used to dispense Rate 5's minimum.
Manifold
Flow/Minimum
Volume
Rate 1 Rate 2 Rate 3 Rate 4 Rate 5
6-Well Flow 560 836 1116 1400 1680
Min Vol 40 80 200 240 320
12-Well Flow 420 627 837 1050 1260
Min Vol 30 60 140 160 220
24-Well Flow 280 418 558 700 840
Min Vol 20 40 100 120 160
48-Well Flow 279 372 560 600 800
Min Vol 40 80 200 240 320
MultiFlo™ FX Multi-Mode Dispenser