Biotek MultiFlo FX - Page 145

Predefined Sample Protocols | 123
P-384_
DILUTION
Serial dilution protocol dispenses 2 µL to 94 µL in 2 µL increments to
each column of the plate. 2 µL to column 1; 4 µL to column 2; and so on
till 94 µL to column 24.
S-96_
DILUTION
Syringe dispenser serial dilution protocol dispenses 240 µL to 20 µL in
20 µL increments to each column of the plate. 240 µL to column 1; 220
µL to column 2; 200 µL to column 3; and so on till 20 µL to column 12.
Defined for Syringe A.
S-384_
DILUTION
Serial dilution protocol dispenses 98 µL to 6 µL in 4 µL increments to
each column of the plate. 98 µL to column 1; 94 µL to column 2; 90 µL
to column 3; and so on till 6 µL to column 24. Defined for Syringe A.
Cell Wash
W&P-96_
CELL_WASH
Cell wash-dispense protocol uses the special low-flow tubing, optimal
dispense and aspirate heights, and vacuum delay during the wash step.
Following the wash, the Peri-pump dispenses 200 µL to each well.
Microplate Manufacturers
W-
COSTARFLAT
Standard wash protocols modified to best position the manifold tubes
for dispensing and aspirating to Corning Costar flat-bottomed and
round-bottomed wells.
W-COSTAR_
ROUND
W-NUNC_384 Standard wash protocols modified to best position the manifold tubes
for dispensing and aspirating to Nunc
®
flat-bottomed wells, round-
bottomed wells, and 384-well plates.
W-NUNC_FLAT
W-NUNC_
ROUND
RAD Technology Peri-pump Cassettes
P_RAD-96 100 µL/well Peri-pump dispense using a single-tube RADcassette to
partially fill a 96-well plate, hit-picking wells to spell "RAD." Begins with
two 20 µL/tube pre-dispenses.
P_RAD-384 50 µL/well Peri-pump dispense using a single-tube RADcassette to
partially fill a 384-well plate, hit-picking wells in a pattern that
represents the BioTek logo. Begins with two 10 µL/tube pre-dispenses.
P_RAD 8Tube-
24
1120 µL/well Peri-pump dispense using the 8-to-1 RADcassette to
partially fill a 24-well plate, dispensing to each corner well and 4 wells in
the center. Tip Tracking is enabled with an initial Z-axis of 5.08 mm. The
chute is positioned to direct the fluid stream to the well walls with an X-
axis offset of 2.97 mm.
Note: duplicate protocols defined for the Secondary Peri-pump have a P2 prefix.
BioTek Instruments, Inc.