132 | Chapter 3: Operation
14 3
15 3
16 2
17 2
18 2
19 2
20 2
21 1
22 1
23 1
24 1
25 1
Alternatively, Use Nth Plate steps to create mixed groups of plates, where each
group contains a plate with 5 wash cycles, a plate with 4 wash cycles, a plate with 3
wash cycles, and so on.
Serial Dilutions Examples
Nth Plate: To generate mixed groups of dilutions in the stack of plates, as depicted
in the table below, three Nth Plate steps are used. The entire stack can be processed
this way.
1st Nth Plate: Every 3rd plate starting with 1
2nd Nth Plate: Every 3rd plate starting with 2
3rd Nth Plate: Every 3rd plate starting with 3
Plate Volume
1 100 µL
2 200 µL
3 300 µL
4 100 µL
5 200 µL
6 300 µL
7 100 µL
8 200 µL
9 300 µL
10 100 µL
11 200 µL
12 300 µL
MultiFlo™ FX Multi-Mode Dispenser