Biotek MultiFlo FX - Page 231

Long Shutdown (Prepare for Storage or Shipment) | 209
Predefined protocols are installed onboard the instrument and on the host
computer during installation of the LHC:
o
W-LONG_SHUTDOWN — for the washer
o
S-LONG_SHUTDOWN — for the Syringe dispenser
The LONG_SHUTDOWN protocols flushes and soaks the supply and manifold tubing
with disinfectant, then flushes with rinse, and finally purges the system of fluid.
For the Syringe dispensers: copy this protocol and modify it to run on Syringe B.
n Three supply bottles are required for this procedure: one for disinfectant, one
for rinse, and one for air.
Caution!Be sure to check the percentage NaClO of the bleach you are using; this
information is printed on the side of the bottle. Commercial bleach is typically 10%
NaClO; in this case, prepare a 1:20 dilution. Household bleach is typically 5% NaClO; in
this case, prepare a 1:10 dilution.
1. Turn the instrument off and disconnect the power cord.
2. Unload the Peri-pump cassette and the prime trough inserts, if applicable.
Clean and store them separately.
3. Empty the waste bottle.
4. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach).
5. Fill one supply bottle with at least 400 mL of bleach solution (disinfectant).
6. Fill another supply bottle with at least 800 mL of deionized water (rinse).
7. Keep the third supply bottle empty (air).
8. Turn on the instrument and run W-LONG_SHUTDOWN.
n While this program is running, you will need to periodically check the display
panel and follow the instructions.
9. When the washer has been cleaned and purged, repeat the process to clean and
purge the Syringe dispensers, if applicable:
o
Prepare the supply bottles with disinfectant, rinse and air;
o
Run S-LONG_SHUTDOWN
BioTek Instruments, Inc.