Calibrate the Backlash for Syringe Dispenser | 213
4. Upon completion, carefully remove and reweigh the plate to determine the
Actual Weight.
5. The Expected Weight is:
o
16 tube = 7.680 grams
o
32 tube = 9.216 grams
o
8 tube = 1.920 grams
6. Calculate the volume (weight) error and the backlash adjustment that needs to
be made:
o
16 tube: (7.680 - Actual Weight ÷ 24 ÷ [.0033 or .0031*]) = backlash setting
o
32 tube: (9.216 - Actual Weight ÷ 48 ÷ [.0033 or .0031*]) = backlash setting
o
8 tube: (1.920 - Actual Weight ÷ 12 ÷ [.0033 or .0031*]) = backlash setting
* use .0033 for autoclavable units; use .0031 for non-autoclavable units.
l Subtract the Actual Weight from the Expected Weight and divide by
the number of columns dispensed to.
l Divide the result by 0.0033 and round to the nearest whole number.
Example: 7.680 - 7.550 = 0.130, 0.130/24 = 0.00542, 0.00542/0.0033 = 1.64
rounded to 2. The backlash setting needs to be adjusted by 2.
7. Adjust the backlash as necessary: select Tools>Instrument Utilities> Syringe
Dispenser and enter the number of steps in the applicable Backlash fields in
the Calibration Data group box.
8. Repeat steps 1 through 4 until the volume dispensed is within one backlash
unit of being exact: ± 0.119 mL.
BioTek Instruments, Inc.
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