8 | Chapter 1: Introduction
Device
Volume
range
µL/well
Precision Accuracy
Approximate Dead
volume
/48-well certain
µL/well
any
µL/well
Syringe 32-tube 3-3000 <12% CV
@ 6
µL/well
±5% @ 6
µL/well
Strip Washer 20 –
30,000
≤3.0 - 5.0
% CV
¥
≤±3.0% 12 mL
*1 µL cassettes' maximum recommended dispense volume is 50 µL/well.
‡ 0.5 µL dispensing is supported when using a 1 µL cassette.
¥Depending on wash manifold, See Performance Specifications on page 21.
BioTek recommends priming a dispenser with three times its dead volume to
prepare it for accurate dispensing.
Processing Time
§
Protocols were optimized for speed to obtain the following processing times,
including the fastest flow and travel rates. Some of these parameters are listed in
the Parameters column of the table. Only standard, non-RAD Peri-pump
cassettes are referenced here.
Device Plate
Type
Volume
(µL/well)
Parameters Time in
seconds
¥
Peri-pump - 5 µL 96 10 High flow rate 3
Peri-pump - 1 µL 384 1 High flow rate 6
1536 1 High flow rate 21
Syringe 8-tube 96 20 Flow rate 1 6.5
Syringe 16-tube 96 20 Flow rate 1 5.25
384 20 Flow rate 1 14
Syringe 32-tube 1536 3 One SB manifold 16.5
1536 14 One LB manifold 27
Strip Washer 96 300 3 cycles <105
§ Review the Specifications for more details.
¥ Excluding plate carrier and manifold homing movements.
SB = small bore Syringe manifold; LB = large bore manifold.
MultiFlo™ FX Multi-Mode Dispenser