226 | Chapter 5: Qualification
10. Read the plate in an absorbance reader using the dual-wavelength method: read
the plate at 630 nm and 450 nm.
11. Calculate the Delta OD: (630 nm - 450 nm), Mean Absorbance, Standard
Deviation, and the %CV for the wells under test. %CV = (Standard Deviation ÷
Mean) * 100.
12. Print the report, obtain required signatures, and store it according to regulatory
guidelines.
If one or more of your tests are failing, make sure the dispense tubes are not
clogged, (follow instructions to Unclog the Dispense Tips on page 194). If that doesn’t
work, recalibrate the cassette and repeat the test(s). If your tests continue to fail,
contact BioTek’s Technical Assistance Center (TAC).
Documenting Test Results
Dispense Precision & Accuracy Test Worksheets are provided below. We
recommend you make copies of the appropriate pages and use them to record your
calculations and test results.
Alternatively, you can purchase the instrument qualification package, which
contains additional tools for conducting test procedures and recording the results,
including logbooks and Excel® spreadsheets.
MultiFlo™ FX Multi-Mode Dispenser
To Next Page
Loading...
