9 • DP/SP Service
Doc. 065031-04 1/08 261
9.3.2 Priming from the Control Panel
1. Gradient pump only: Set the channel to be primed (A, B, C, or D) to
100% on the pump Control panel in Chromeleon or Chromeleon
Xpress.
2. Under Prime Control on the Control panel:
a. Set the Duration to 300 sec.
b. Set the Prime Rate to 6.0 mL/min.
3. Open the priming valve on the secondary pump head (see Figure 9-1
)
by turning it one-half turn counterclockwise.
NOTE If the priming valve is opened too much, air is
drawn through the valve and air bubbles can be
seen exiting the waste line.
4. To enable priming, click the Prime button on the Control panel.
5. Continue priming the pump until all air and previous eluents are
purged and no air bubbles can be seen exiting the waste line.
6. Gradient pump only:
a. If all eluent lines have not been primed, select another eluent
channel and repeat the priming procedure.
b. After priming all eluent lines, click the Prime button to stop
priming.
c. Close the priming valve by turning it clockwise. Do not
overtighten the priming valve.
7. Enter the Flow rate required for your application on the Control
panel.
8. Gradient pump only: Enter the required proportions of eluents A, B,
C, and D on the Control panel (under Gradient Control).
9. Click Motor on the Control panel to turn on the pump flow.
Do not use any tools to tighten the priming valve! Overtightening may
destroy the cap seal. Open or close the priming valve only when the
system pressure is down.
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