Thermo Scientific Applied Biosystems SeqStudio Genetic Analyzer - Manage Analysis Settings

b.
Enter a name and select a dye color, then edit the fragment sizes (basepairs).
c.
Click Save.
3.
As needed, select a size standard of interest, then click Edit or Delete
(user-created size standards only).
Manage analysis settings
In the Properties tab:
1.
Select Actions4Analysis seings.
2.
To create new analysis seings:
a.
Select the default analysis seings or user-created analysis seings, then
click Copy.
b.
Enter a name and edit seings as needed (see “Fragment/HID analysis
seings (size calling)“ on page 126 or “Sequencing seings (base calling)“ on
page 129).
c.
Click Save.
3.
As needed, select an analysis seing of interest, then click Edit or Delete
(user-created seings only).
Setting
Description
Size calling method
• Local Southern— (default) Determines the fragment sizes using the reciprocal
relationship between fragment length and electrophoretic mobility.
• Global Southern—Compensates for standard fragments with anomalous
electrophoretic mobility (similar to least squares methods).
• 2nd LSQ (2nd Order Least Squares)—Uses regression analysis to build a bestfit
size calling curve.
• 3rd LSQ (3rd Order Least Squares)—Uses regression analysis to build a bestfit
size calling curve.
• Cubic Spline Interpolation—Forces the sizing curve through all the known points
of the selected size standard.
Fragment/HID
analysis settings
(size calling)
Chapter 9 Manage the software (Plate Manager)
Manage analysis settings
9
126
SeqStudio
™
Genetic Analyzer Instrument and Software User Guide